Felix Prado

Research interest

Faithful replication of the complete genome is essential for preventing any loss of genetic information. However, this is not an easy task; in fact, the genetic instability that accompanies tumor progression during early stages is associated with replicative stress. Using the yeast Saccharomyces cerevisiae as living model, and a wide repertoire of techniques in genetic, biochemistry, genomic, and molecular and cellular biology, we are focused on two different aspects associated with replication dynamics that have a direct impact on genome integrity and cell cycle progression: chromatin assembly and tolerance to replicative DNA damage. Our main goal is to get a deeper insight into their mechanistic and regulation, as well as in the consequences of their misregulation for cell fitness.

Publications

  • F. Prado, and D. Maya. 2017. Regulation of replication fork advance and stability by nucleosome assembly. Genes in press

  • F. Prado, S. Jimeno-González and J. C. Reyes. 2016. Histone availability as a strategy to control gene expression. RNA Biology in press

  • S. Jimeno-González, L. Payán-Bravo, A. M. Muñoz-Cabello, M. Guijo, G. Gutierrez, F. Prado and J. C. Reyes. 2015. Defective histone supply causes changes in RNA polymerase II elongation rate and cotranscriptional pre-mRNA splicing. PNAS 112:14840-14845

  • M. Murillo-Pineda, M. J. Cabello-Lobato, M. Clemente-Ruíz, F. Monje-Casas and F. Prado. 2014. Defective histone supply causes condensin-dependent chromatin alterations, SAC activation and chromosome decatenation impairment. Nucleic Acid Research 42:12469-12482

  • F. Prado. 2014. Homologous recombination maintenance of genome integrity during DNA damage tolerance. Molecular and Cellular Oncology e957039

  • F. Prado. 2014. Genetic instability is prevented by Mrc1-dependent spatio-temporal separation of replicative and repair activities of homologous recombination. BioEssays 36: 451-462

  • R. Gonzalez-Prieto, A. Muñoz-Cabello, M. J. Cabello-Lobato and F. Prado. 2013. Rad51 replication fork recruitment is required for DNA damage tolerance. EMBO Journal 32: 1307-1321

  • M. Clemente-Ruiz, R. Gonzalez-Prieto and F. Prado. 2011. Histone H3K56 acetylation, CAF1 and Rtt106 coordinate nucleosome assembly and stability of advancing replication forks. PloS Genetics 7: e1002376

  • M. Morillo-Huesca, M. Clemente-Ruiz, E. Andujar and F. Prado. 2010. In the Absence of H2A.Z the SWR1 Histone Replacement Complex Causes Genetic Instability and Genome-Wide Transcription Misregulation. PloS One 5: e12143

  • M. Clemente and F. Prado. 2009. Chromatin assembly controls replication fork stability. EMBO reports (2009) 10: 790-796

Active Grants

  • 2016-2018. Dinámica de la cromatina asociada a la replicación y estabilidad genómica. Ministerio de Economía y Competitividad BFU2015-63688-P. Investigador responsable: Félix Prado

  • 2016-2017. Dinámica y estabilidad Cromosómica. Ministerio de Economía y Competitividad BFU2015-69142-REDT (Redes de Excelencia). Investigador responsable: Félix Prado

  • 2014-2016. Efecto de la reducción de los niveles de histonas en la estabilidad genética, el envejecimiento celular y la entrada en senescencia. Junta de Andalucia “Proyectos de Excelencia” P12-CTS-2270. Investigador responsable: Félix Prado

Group members

Group leader:
  • Felix Prado Velasco
Staff Scientist:
  • Dr. Macarena Morillo Huesca
Postdoctorals:
  • Douglas Maya Miles
PhD students:
  • Marta Barrientos Moreno
  • María Isabel Cano Linares
  • Aurora Yáñez Vilches
Technicians:
  • Elena Gomez Marin
Master students:
  • Luz Maria Lopez Ruiz