Miguel González Blanco

Research interest

Every time cells divide, the preservation, duplication and transmission of their genetic information must be accurately accomplished. In our lab, we study how cells coordinate the repair of DNA damage with major molecular events like DNA replication and chromosome segregation. With a particular focus on homologous recombination, we currently employ a multi-pronged approach that includes biochemistry, yeast genetics and cell biology to focus on the following questions: i) how the biochemical properties of Holliday junction resolvases can bias repair outcome (crossovers vs non-crossovers); ii) how post-translational modifications regulate the biological functions of DNA repair enzymes and iii) how the misregulation of DNA repair activities may interfere with other cellular processes.

Publications

  • Princz LN, Wild P, Bittmann J, Aguado FJ, Blanco MG, Matos J, Pfander B. Dbf4-dependent kinase and the Rtt107 scaffold promote Mus81-Mms4 resolvase activation during mitosis. EMBO J. 2017 Jan 17. pii: e201694831. doi: 10.15252/embj.201694831. [Epub ahead of print] PubMed PMID: 28096179.

  • Duda H, Arter M, Gloggnitzer J, Teloni F, Wild P, Blanco MG, Altmeyer M, Matos J. A Mechanism for Controlled Breakage of Under-replicated Chromosomes during Mitosis. Dev Cell. 2016 Dec 19;39(6):740-755. doi: 10.1016/j.devcel.2016.11.017. PubMed PMID: 27997828.

  • Blanco MG, Matos J. Hold your horSSEs: controlling structure-selective endonucleases MUS81 and Yen1/GEN1. Front Genet. 2015 Jul 30;6:253. doi: 10.3389/fgene.2015.00253. Review. PubMed PMID: 26284109; PubMed Central PMCID: PMC4519697.

  • Blanco MG, Matos J, West SC. Dual control of Yen1 nuclease activity and cellular localization by Cdk and Cdc14 prevents genome instability. Mol Cell. 2014 Apr 10;54(1):94-106. doi: 10.1016/j.molcel.2014.02.011. PubMed PMID: 24631285; PubMed Central PMCID: PMC3988869.

  • Matos J, Blanco MG, West SC. Cell-cycle kinases coordinate the resolution of recombination intermediates with chromosome segregation. Cell Rep. 2013 Jul 11;4(1):76-86. doi: 10.1016/j.celrep.2013.05.039. PubMed PMID: 23810555.

  • Muñoz-Galván S, Tous C, Blanco MG, Schwartz EK, Ehmsen KT, West SC, Heyer WD, Aguilera A. Distinct roles of Mus81, Yen1, Slx1-Slx4, and Rad1 nucleases in the repair of replication-born double-strand breaks by sister chromatid exchange. Mol Cell Biol. 2012 May;32(9):1592-603. doi: 10.1128/MCB.00111-12. PubMed PMID: 22354996; PubMed Central PMCID: PMC3347241.

  • Matos J, Blanco MG, Maslen S, Skehel JM, West SC. Regulatory control of the resolution of DNA recombination intermediates during meiosis and mitosis. Cell. 2011 Sep 30;147(1):158-72. doi: 10.1016/j.cell.2011.08.032. PubMed PMID: 21962513; PubMed Central PMCID: PMC3560330.

  • Rass U, Compton SA, Matos J, Singleton MR, Ip SC, Blanco MG, Griffith JD, West SC. Mechanism of Holliday junction resolution by the human GEN1 protein. Genes Dev. 2010 Jul 15;24(14):1559-69. doi: 10.1101/gad.585310. PubMed PMID: 20634321; PubMed Central PMCID: PMC2904945.

  • Blanco MG, Matos J, Rass U, Ip SC, West SC. Functional overlap between the structure-specific nucleases Yen1 and Mus81-Mms4 for DNA-damage repair in S. cerevisiae. DNA Repair (Amst). 2010 Apr 4;9(4):394-402. doi: 10.1016/j.dnarep.2009.12.017. PubMed PMID: 20106725.

  • Ip SC, Rass U, Blanco MG, Flynn HR, Skehel JM, West SC. Identification of Holliday junction resolvases from humans and yeast. Nature. 2008 Nov 20;456(7220):357-61. doi: 10.1038/nature07470. PubMed PMID: 19020614.

Active Grants

  • Biochemical analysis of the processing of DNA replication and recombination intermediates. PI: Miguel González Blanco. BFU2016-78121-P. Programa Estatal de Fomento de la Investigación, Científica y Técnica de Excelencia, Ministerio de Economía y Competitividad. 30/12/2016-29/12/2019. Budget: 181.500 €.

  • Consolidación e estruturación - 2016 GPC GI-2119, Mecanismos Moleculares de Enfermidade (MeMoEn). Coordinator: Juan Bautista Zalvide Torrente (Total PIs: 6). Proxectos Plan Galego IDT, Xunta de Galicia (ED431B 2016/016). 01/01/2017-30/11/2018. Budget: 70.000 €.

  • Consolidación e estruturación - modalidade D 2016. PI: Miguel González Blanco. Proxectos Plan Galego IDT, Xunta de Galicia (ED431F 2016/019). 01/01/2016-30/11/2019. Budget: 90.000 €.

  • “Ramón y Cajal” start-up funding. PI: Miguel González Blanco. RYC-2012-10835. Ministerio de Economía y Competitividad. 01/09/2014-31/08/2019. Budget: 40.000 €.

Group members

  • Francisco Javier Aguado Domínguez (PhD student)
  • Raquel Carreira Rodríguez (MSc student)
  • Miguel González Blanco (PI)
  • Diana Guallar Artal (Postdoctoral researcher)
  • Tomás Lama Díaz (MSc student)
  • Vanesa Hurtado Nieves (PhD student)
  • Paula Sánchez Mandayo (Undergraduate student)