Travis Stracker

DNA replication and Genome Integrity maintenance
http://www.irbbarcelona.org/tstracker

Research interest

My laboratory examines molecular mechanisms that promote genome stability and their role in hereditary disease pathology and cancer predisposition. We also investigate potential targets that may augment current chemotherapeutic approaches. Our current efforts focus on the Tousled like kinases 1 and 2 that regulate the histone chaperone ASF1, and potentially other yet unidentified substrates. We have found that these kinases play important roles in genome maintenance and we are taking genome wide sequencing and proteomics approaches to understand their function.

Publications

  • Segura-Bayona S, Knobel PA, González Burón H, Youssef SA, Peña-Blanco A, Coyaud E López-Rovira T, Rein K, Palenzuela L, Colombelli J, Forrow S, Raught B, Groth A, de Bruin A, Stracker TH. Differential requirements for Tousled like kinases 1 and 2 in mammalian development (manuscript under revision).

  • Vinaixa M, Rodríguez MA, Aivio S, Capellades J, Gómez J, Canyellas N, Stracker TH, Yanes O. Positional Enrichment by Proton Analysis (PEPA): A One-Dimensional 1 H-NMR Approach for 13 C Stable Isotope Tracer Studies in Metabolomics. Angew Chem Int Ed Engl. 2017 Mar 20;56(13):3531-3535. doi: 10.1002/anie.201611347. Epub 2017 Feb 21. PMID: 28220994

  • Navarro J, Gozalbo-López B, Méndez AC, Dantzer F, Schreiber V, Martínez C, Arana DM, Farrés J, Revilla-Nuin B, Bueno MF, Ampurdanés C, Galindo-Campos MA, Knobel PA, Segura-Bayona S, Martin-Caballero J, Stracker TH, Aparicio P, Del Val M, Yélamos J. PARP-1/PARP-2 double deficiency in mouse T cells results in faulty immune responses and T lymphomas. Sci Rep. 2017 Feb 9;7:41962. doi: 10.1038/srep41962. PMID: 28181505

  • Terre B, Piergiovanni G, Segura-Bayona S, Gil-Gomez G, Jung C, Youssef S, Marjanovic M, Knobel PA, Palenzuela L, Lopez-Rovira T, Forrow S, Stephan Otto-Attolini C, Valverde M, de Bruin A, Costanzo V and Stracker TH. GEMC1 is an apical regulator of multiciliated cell differentiation. EMBO J. 2016 May 2;35(9):942-60. doi: 10.15252/embj.201592821. Epub 2016 Mar 1. PMID: 26933123

  • Pereira-Lopes S, Tur J, Calatayud-Subias JA, Lloberas J, Stracker TH, Celada A. NBS1 is required for macrophage homeostasis and functional activity in mice. Blood. 2015 Nov 26;126(22):2502-10. doi: 10.1182/blood-2015-04-637371. Epub 2015 Aug 31. PMID: 26324700

  • Rein K, Yanez DA, Terre B, Palenzuela L, Aivio S, Wei K, Edelmann W, Stark JM, Stracker TH. EXO1 is critical for embryogenesis and the DNA damage response in mice with a hypomorphic Nbs1 allele. Nucleic Acids Res. 2015 Jul 8. pii: gkv691. [Epub ahead of print] PMID: 26160886

  • Marjanović M, Sánchez-Huertas C, Terré B, Gómez R, Scheel JF, Pacheco S, Knobel PA, Martínez-Marchal A, Aivio S, Palenzuela L, Wolfrum U, McKinnon PJ, Suja JA, Roig I, Costanzo V, Lüders J, and Stracker TH. CEP63 deficiency promotes p53-dependent microcephaly and reveals a role for the centrosome in meiotic recombination. Nat Commun. 2015 Jul 9;6:7676. doi: 10.1038/ncomms8676. PMID: 26158450

  • Chen WT, Ebelt ND, Stracker TH, Xhemalce B, Van Den Berg CL, Miller KM. ATM regulation of IL-8 links oxidative stress to cancer cell migration and invasion. Elife. 2015 Jun 1;4. doi: 10.7554/eLife.07270. [Epub ahead of print] PMID: 26030852

  • Arroyo R, Suñé G, Zanzoni A, Duran-Frigola M, Alcalde V, Stracker TH, Soler-López M, Aloy P. Systematic identification of molecular links between core and candidate genes in breast cancer. J Mol Biol. 2015 Mar 27;427(6 Pt B):1436-50. doi: 10.1016/j.jmb.2015.01.014. Epub 2015 Jan 29. PMID: 25640309

  • Rein K, Stracker TH. The MRE11 complex: an important source of stress relief. Exp Cell Res. 2014 Nov 15;329(1):162-9. doi: 10.1016/j.yexcr.2014.10.010. Epub 2014 Oct 18. Review. PMID: 25447316

  • Zhen Y, Knobel PA, Stracker TH, Reverter D. Regulation of USP28 deubiquitinating activity by SUMO conjugation. J Biol Chem. 2014 Dec 12;289(50):34838-50. doi: 10.1074/jbc.M114.601849. Epub 2014 Oct 30. PMID: 25359778

  • Torres AG, Piñeyro D, Filonava L, Stracker TH, Batlle E, Ribas de Pouplana L. A-to-I editing on tRNAs: biochemical, biological and evolutionary implications. FEBS Lett. 2014 Nov 28;588(23):4279-86. doi: 10.1016/j.febslet.2014.09.025. Epub 2014 Sep 27. Review. PMID: 25263703

  • Knobel PA, Belotserkovskaya R, Galanty Y, Schmidt CK, Jackson SP, Stracker TH. USP28 is recruited to sites of DNA damage by the tandem BRCT domains of 53BP1 but plays a minor role in double-strand break metabolism. Mol Cell Biol. 2014 Jun;34(11):2062-74. doi: 10.1128/MCB.00197-14. Epub 2014 Mar 31. PMID: 24687851

  • Bianchi J, Rudd SG, Jozwiakowski SK, Bailey LJ, Soura V, Taylor E, Stevanovic I, Green AJ, Stracker TH, Lindsay HD, Doherty AJ. PrimPol bypasses UV photoproducts during eukaryotic chromosomal DNA replication. Mol Cell. 2013 Nov 21;52(4):566-73. doi: 10.1016/j.molcel.2013.10.035. PMID: 24267451

  • Ballew BJ, Joseph V, De S, Sarek G, Vannier JB, Stracker TH, Schrader KA, Small TN, O'Reilly R, Manschreck C, Harlan Fleischut MM, Zhang L, Sullivan J, Stratton K, Yeager M, Jacobs K, Giri N, Alter BP, Boland J, Burdett L, Offit K, Boulton SJ, Savage SA, Petrini JH. A recessive founder mutation in regulator of telomere elongation helicase 1, RTEL1, underlies severe immunodeficiency and features of Hoyeraal Hreidarsson syndrome. PLoS Genet. 2013 Aug;9(8):e1003695. doi: 10.1371/journal.pgen.1003695. Epub 2013 Aug 29. PMID: 24009516

  • Brown NJ, Marjanović M, Lüders J, Stracker TH, Costanzo V. Cep63 and cep152 cooperate to ensure centriole duplication. PLoS One. 2013 Jul 30;8(7):e69986. doi: 10.1371/journal.pone.0069986. Print 2013. PMID: 23936128

  • Stracker TH, Roig I, Knobel PA, Marjanović M. The ATM signaling network in development and disease. Front Genet. 2013 Mar 25;4:37. doi:10.3389/fgene.2013.00037. eCollection 2013. PMID: 23532176

  • Foster SS, De S, Johnson LK, Petrini JH, Stracker TH. Cell cycle- and DNA repair pathway-specific effects of apoptosis on tumor suppression. Proc Natl Acad Sci U S A. 2012 Jun 19;109(25):9953-8. doi: 10.1073/pnas.1120476109. Epub 2012 Jun 5. PMID: 22670056

Active Grants

  • BFU2015-68354

Group members

Group leader:
  • Travis Stracker
Postdoctorals:
  • Philip Knobel
  • Isabel Garcia-Cao
  • Ilaria Dutto
  • Berta Terre
PhD students:
  • Sandra Segura-Bayona
  • Marina Villamor
  • Joana Silva
Technicians:
  • Lluis Palenzuela